绵羊慢病毒自然感染绵羊的硬化性淋巴细胞性乳腺炎

７头来自新疆南部某绵羊慢病毒（ＯｖＬＶ）感染的羊场的绵羊用于本研究。用琼脂凝胶免疫扩散检查绵羊血清中对绵羊进行性肺炎（ＯＰＰ）病毒（ＯＰＰＶ）的抗体，结果表明有６例呈阳性，１例阴性，抗体效价在３年中呈下降趋势。４例血清学阳性边菜羊和１例阴性和田羊有不同程度的硬化性（纤维性）淋巴细胞性乳腺炎，小叶内有不等的淋巴细胞浸润，导管周围无淋巴滤泡形成，小叶间大量纤维组织增生。７例的肺、脑、关节、血管均无ＯｖＬＶ性特异性病变。从血清学阳性羊的外周血白细胞中未分离到ＯｖＬＶ。     

猪传染性胃肠炎病毒南京株纤突S蛋白基因的克隆与序列分析

参考 Genbank收录的 TGEV- Miller株的基因序列 ,自行设计合成 1对引物 (TGEVP5 /P6 ) ,对不同代次的 TGEV疫苗弱毒 STC3及种毒 、种毒 进行了 RT- PCR扩增 ,产物经琼脂糖凝胶电泳分析 ,均出现 1条大约 12 6 2 bp的目的条带 ,经 Eco R 酶切 ,都产生了 871bp和391bp左右的两个片段 ,与预期大小相符。将种毒 RT- PCR扩增目的条带回收纯化后克隆入PMD18- T载体中 ,转化宿主菌 DH5 α,挑选阳性克隆 (命名为 PTs) ,提取重组质粒 ,用 Hpa 、Eco R 对重组质粒进行酶切鉴定以及 PCR扩增 ,然后进行序列测定 ,并进行了序列分析 ,证实与国外标准毒株 Miller、Fs772 / 70、Purdue、TO14等有较高的同源性     

单纯疱疹病毒脑炎模型的研究

对单纯疱疹病毒(HSV)感染小鼠致病特点进行了观察,小鼠感染HSV第3天后开始发病,感染后第2天脑内可检测出病毒核酸,第3天脑内可分离出病毒,第4天病毒滴度达到高峰,发病2天内死亡。电镜观察感染鼠脑内组织出现超微结构改变,并可找到病毒颗粒。结果说明所建立的HSV脑炎模型可用于评价抗HSV药物。     

赤羽病微量病毒中和试验诊断方法的研究

应用引自日本的赤羽病病毒，制备了微量病毒中和试验抗原和高免阳性血清，建立了赤羽病微量病毒中和试验诊断方法，应用此方法对广东省７２４头牛血清进行了检查，阳性率为３５．３％，对上海６６５头牛血清作了检查，阳性率为６７．７％，说明这些地区曾流行过赤羽病，同时对澳大利亚当地牛１０８头进行检查，阳性率为５５．５％，与外报道一致。对澳大利亚、加拿大，美国进口牛１７３头的检查结果，全部阴性，对澳大利亚进口羊９９２头的检查结果，发现阳性１头，已得到澳方认可。     

应用生物素标记的NDV－cDNA探针检测感染尿囊液中NDV－RNA的初步研究

本文应用聚合酶链反应（ＰＣＲ）技术从构建的新城疫病毒（ＮＤＶ）ｃＤＮＡ文库中扩增含编码Ｆ糖蛋白前体──Ｆｏ酶切位点序列的３５９ｂｐ的Ｆ蛋白基因ｃＤＮＡ片段。将此３５９ｂｐｃＤＮＡ片段经光敏生物素标记后,即成ＮＤＶ－ｃＤＮＡ探针。该探针能特异性地从感染的尿囊液中检测出ＮＤＶ强毒株和疫苗毒株的基因组ＲＮＡ,而不与ＩＢＤｖ－ｄｓＲＮＡ、ＡＩＢｖ－ｓｓＲＮＡ、ＥＤＳ７６－ｄｓＤＮＡ、ＭＤＶ－ｄｓＤＮＡ,ＦＰＶ－ｄｓＲＮＡ及ＡＩＬＶ－ｄｓＤＮＡ发生交叉杂交反应。试验结果表明：尽管该探钎含有编码Ｆｏ蛋白酶切位点序列的碱基顺序,但它还是不能把ＮＤＶ的强、弱毒株区分开。这说明ＮＤＶ强、弱毒株比区域内的碱基存在着相当大的同源性。不过,此探针对ＮＤＶ来说具有特异性,这就为ＮＤＶ的诊断技术开创了基因水平检测的新途径。     

用酶联免疫吸附试验检测鸡白血病以及控制、根除鸡白血病措施的探讨

用酶联免疫吸附试验(ELISA),对2群鸡的鸡蛋清和1株鸡的马立克氏病疫苗进行检测,发现2群鸡的鸡蛋清中,鸡白血病病毒的阳性率分别是11％和29％,鸡马立克氏病冻干苗隐藏鸡白血病病毒群体特异性(gs)抗原的阳性率为100％。本文指出我国禽苗可能带有鸡的白血病病毒,分析讨论了鸡白血病病毒的垂直传递和水平传播的规律,提出在曾祖代和祖代鸡群中,采用ELISA试验,检测鸡蛋清,能减少以至根除鸡的白血病。     

Identification of blackeye cowpea mosaic virus from germplasm of yard-long bean and from soybean,and the relationships between blackeye cowpea mosaic virus and cowpea aphid-borne mosaic virus

Two potyvirus isolates, one from germplasm of yard-long bean (Vigna unguiculata ssp.sesquipedalis) introduced into the Netherlands, and another one from soybean plants (Glycine max) in Indonesia, were compared with two virus isolates of blackeye cowpea mosaic virus (BICMV) from the USA and a Moroccan isolate of cowpea aphid-borne mosaic virus (CAMV). It is proposed that all five isolates be now considered BICMV on the basis of host ranges, symptoms and serology. From our results, and a reassessment of the literature it is suggested to drop the name CAMV in favour of BICMV.Samenvatting Twee potyvirussen, de een in Nederland ingevoerd met genenmateriaal vanVigna unguiculata ssp.sesquipedalis en de ander uit planten van sojaboon (Glycine max) in Indonesië, werden vergeleken met twee isolaten van blackeye cowpea mosiac virus (BICMV) en een Marokkaans isolaat van cowpea aphid-borne mosaic virus (CAMV). Op grond van waardplantenreeksen, symptomen en serologie stellen de auteurs voor om alle vijf isolaten te beschouwen als BICMV. Gebaseerd op de verkregen resultaten en een kritische beschouwing van de literatuur wordt de aanbeveling gedaan om de naam CAMV te laten vallen ten gunste van BICMV.     

Transmission of the agent causing a melon yellowing disease by the greenhouse whitefly Trialeurodes vaporariorum in southeast Spain

The agent causing a yellowing disease of melon (Cucumis melo), which results in severe losses in crops under plastic on the coastal plains of southeast Spain, was shown to be transmitted in a semipersistent manner by the greenhouse whitefly (Trialeurodes vaporariorum Westwood). The agent was transmitted by grafting, but not by mechanical inoculation or through seeds. The agent was acquired in the minimum period tested (2 h) and could infect plants in an infection feeding interval of 6 h. Capsella bursa-pastoris, Cucumis melo, C. sativus, Cucurbita moschata, Cichorium endivia, Lactuca sativa andTaraxacum officinale were found susceptible.Results suggest that the yellowing disease affecting melon crops in the southeast of Spain is due to a pathogen similar to beet pseudo yellows virus, but this has to be confirmed by serology.     

家蚕质型多角体病毒苏州核包涵体小种的纯化

从BmCPV_t内发现和分离出一株新的核包涵体小种,初步命名为家蚕质型多角体病毒—苏州核包涵体小种(Cytoplasmic Polyhedrosis Virus——Suzhou nuclear polyhedra forming strain,简称CPV——Suzhou N strain),家蚕感梁CPV——Suzhou N Strain引起的多角体病,与国内外已知的CPV各变异株不同。本病蚕的多角体只在中肠组织圆筒形细胞核内检出,多角体内有许多病毒粒子,病毒粒子球状,具有核蛋白紫外吸收特征性光谱和较强的感染活性,病毒核酸初步鉴定为dsRNA。     

Comparative studies of strains of infectious bovine rhinotracheitis virus isolated from latently infected calves

Three strains (479 C, 778 TL, 982 LE) of infectious bovine rhinotracheitis (IBR) virus isolated from latently infected calves were compared with the prototype strain of IBR virus (LA strain) in studies which included restriction endonuclease analysis, experimental infection, and reciprocal cross protection tests in cattle. From the restriction endonuclease analysis it appeared that the 3 "latent" viruses were derived from the same isolate, and that it differed slightly from the LA strain. However, latency does not seem to have affected the pathogenicity or the immunogenicity of the virus. This is demonstrated by the identical clinical and virologic response of calves subjected to experimental infection with the various strains under study, and by the finding that when the LA strain and a "latent" strain (982 LE) were tested in cross protection tests in cattle, they proved to be mutually protective.